Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Enzo Life Sciences Physiological saline (NaCl 0.9%) (endotoxin-free) (sterile) (1.5ml)
Sterile and endotoxin-free physiological saline for use with TLRGRADE™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal. Long Term Storage: +4°C.
ILEX LIFE SCIENCES LLC EDTA 1P P10-026100 6 X 100 ML
PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 1.00% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Teknova 20X PBS with 1% Tween-20. 500mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Growcells PBS 20X 2L PK6
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Aniara Diagnostica LLC PBS TBLT PH 7.4 1000ML 100 EA
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed tablets yields 1000 mL 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Use as biological buffer, isotonic. Bottle of 100 tablets.
PLANT CELL TECHNOLOGY 1X Phosphate Buffered Saline Solution, pH 7.4, without Calcium and Magnesium, Buffer Solution, Mod. 6, Clear Liquid, Sterile, 500mL bottle.
1X PBS Solution Mod. 6, pH 7.4, is a sterile buffer used in mammalian cell culture to maintain physiological pH during washing, dilution, or reagent preparation. Packaged in a clear 500mL bottle. Ready-to-use and stable under recommended conditions. Ideal for research labs, tissue culture, and molecular biology workflows.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.4) - 2x1L
Bioprocess Grade - Phosphate Buffered Saline PBS (10X pH 7 4) - 2x1L (For Research Use and Further Manufacturing Only)
![Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0ml Pre Filled Tubes, Sterile, 36 Tubes per Case, 4.5ml Tube](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660239.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0ml Pre Filled Tubes, Sterile, 36 Tubes per Case, 4.5ml Tube
PBS (Phosphate Buffered Saline) 1X Solution is a sterile, ready-to-use solution supplied in pre-filled 1.0mL tubes for laboratory use. The 36-tube pack is designed for applications requiring quick and convenient aliquots of PBS without the risk of contamination. PBS is essential for maintaining the osmotic balance and pH stability of biological samples during handling, washing, and processing. This product is commonly used in cell culture, microbiological assays, and molecular biology techniques. The pre-filled tube format minimizes handling and preparation time, making it ideal for high-throughput research environments, diagnostic labs, and any setting where reproducibility and precision are crucial.
![Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 4.5mL Pre Filled Tubes, Sterile, 36 Tubes per Case, 10mL Tube](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659891.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 4.5mL Pre Filled Tubes, Sterile, 36 Tubes per Case, 10mL Tube
PBS (Phosphate Buffered Saline) 1X Solution is available in pre-filled 4.5mL tubes, designed for use in laboratory assays requiring higher volumes of buffer. This convenient format eliminates the need for measuring and pouring PBS, reducing the risk of contamination and improving efficiency in laboratory workflows. With 36 tubes per case, it is ideal for high-throughput applications such as cell culture, washing, and sample dilution. PBS ensures that the osmotic pressure and pH of biological systems are maintained, providing stable conditions for cells and biomolecules. This product is widely used in molecular biology, immunology, and microbiology experiments.
Boston Bioproducts Inc Sodium Phosphate Buffer (1 M, pH 7.6) - 500ml
Sodium Phosphate Buffer (1 M pH 7 6) - 500ml (For Research Use and Further Manufacturing Only)
Growcells PBS 20X 2L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g